Issresearch - Blog Posts

Your Gut in Space

Finding the Right Balance for the Microbiota

Trillions of microorganisms live on and in the human body, many of them essential to its function and health. These organisms, collectively known as the microbiota, outnumber cells in the body by at least five times. 

Microorganisms in the intestinal tract, the gut microbiota, play an especially important role in human health. An investigation on the International Space Station, Rodent Research-7 (RR-7), studies how the gut microbiota changes in response to spaceflight, and how that change in turn affects the immune system, metabolic system, and circadian or daily rhythms. 

Research shows that the microbiota in the mammalian digestive tract has a major impact on an individual’s physiology and behavior. In humans, disruption of microbial communities has been linked to multiple health problems affecting intestinal, immune, mental and metabolic systems.

The investigation compares two different genetic strains of mice and two different durations of spaceflight. Twenty mice, ten of each strain, launch to the space station, and another 20 remain on the ground in identical conditions (except, of course, for the absence of gravity). Mice are a model organism that often serves as a scientific stand-in for other mammals and humans. 

Fecal material collected from the mice every two weeks will be examined for changes in the gut microbiota. Researchers plan to analyze fecal and tissue samples after 30 and 90 days of flight to compare the effects of different durations of time in space. 

With a better understanding of relationships between changes such as disruption in sleep and an imbalance of microbial populations, researchers can identify specific factors that contribute to changes in the microbiota. Further studies then can determine proactive measures and countermeasures to protect astronaut health during long-term missions. 

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The As, Gs, Cs and Ts of the Space Station: First In-Space Microbe Identification

Being able to identify microbes in real-time aboard the International Space Station, without having to send them back to Earth for identification first, would be totally amazing for the world of microbiology and space exploration.

The Genes in Space 3 team turned that possibility into a reality this year, when it completed the first-ever sample-to-sequence process entirely aboard the space station.

The ability to identify microbes in space could aid in the ability to diagnose and treat astronauts in real time, as well as assisting in the identification of life on other planets. It could also benefit other experiments aboard the space station.

HELPFUL SCIENCE HINT: Identifying microbes involves isolating the DNA of samples, and then amplifying – or making lots and lots (and LOTS) of copies - of that DNA that can then be sequenced, or identified.  

As part of regular monitoring, petri plates were touched to various surfaces of the space station. NASA astronaut Peggy Whitson transferred cells from growing bacterial colonies on those plates into miniature test tubes, something that had never been done before in space (first OMG moment!).

Once the cells were successfully collected, it was time to isolate the DNA and prepare it for sequencing, enabling the identification of the unknown organisms – another first for space microbiology.

Enter Hurricane Harvey. *thunder booms*

“We started hearing the reports of Hurricane Harvey the week in between Peggy performing the first part of collecting the sample and gearing up for the actual sequencing,” said Sarah Wallace, the project’s primary investigator.

When our Johnson Space Center (JSC) in Houston became inaccessible due hurricane conditions, Marshall Space Flight Center’s Payload Operations Integration Center in Huntsville, Alabama worked to connect Wallace to Whitson using Wallace’s personal cell phone.

With a hurricane wreaking havoc outside, Wallace and Whitson set out to make history.

The data were downlinked to the team in Houston for analysis and identification.

“Once we actually got the data on the ground we were able to turn it around and start analyzing it,” said Aaron Burton, the project’s co-investigator. “You get all these squiggle plots and you have to turn that into As, Gs, Cs and Ts.”

Those As, Gs, Cs and Ts are more than just a nerdy alphabet – they are Adenine, Guanine, Cytosine and Thymine – the four bases that make up each strand of DNA and can tell you what organism the strand of DNA came from. 

“Right away, we saw one microorganism pop up, and then a second one, and they were things that we find all the time on the space station,” said Wallace. “The validation of these results would be when we got the sample back to test on Earth.”

Soon after, the samples returned to Earth aboard the Soyuz spacecraft, along with Whitson.

With the samples now in the team’s JSC lab, tests were completed in ground labs to confirm the findings from the space station. They ran the tests again and again, and then once more, to confirm accuracy. Each time, the results were exactly the same on the ground as in orbit. (second OMG moment!)

“We did it. Everything worked perfectly,” said Sarah Stahl, microbiologist.

This capability could change future space exploration.

“As a microbiologist,” said Wallace, “My goal is really so that when we go and we move beyond ISS and we’re headed towards Mars or the moon or wherever we are headed to, we have a process that the crew can have that great understanding of the environment, based on molecular technology.”

For more information, follow @ISS_Research. 

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One Year Later

On March 1, 2016, veteran astronaut Scott Kelly returned from his Year in Space mission. In many ways, the adventure was just beginning.

The spaceflight part of the One Year Misson to the International Space Station ended a year ago today, but the science behind it is still moving. Astronaut Scott Kelly and Russian cosmonaut Mikhail Kornienko continue to provide samples for the data collection from their ground-breaking mission. Results are expected to to start coming later in 2017, which will help launch humanity on deep space missions.

Kelly not only commanded the International Space Station’s Expedition 46, he participated in spacewalks like this one on Dec. 21, 2015, in which Kelly and astronaut Tim Kopra successfully moved the Space Station's mobile transporter rail car ahead of the docking of a Russian cargo supply spacecraft.

On the station in 2015, Kelly showed off his home away from home. Scott tweeted this image out with the comment: "My #bedroom aboard #ISS. All the comforts of #home. Well, most of them. #YearInSpace." 

Why was the Year In Space important? As we work to extend our reach beyond low-Earth orbit, how the human body reacts to microgravity for extended periods is of paramount importance. Not only were Kelly and his Russian counterpart monitored throughout the mission, they both continue to submit to tests and monitoring one year later to see if there are any lasting effects from their voyage aboard the station. 

Scott Kelly also a human control here on Earth, his identical twin brother and fellow astronaut Mark Kelly. Both brothers have served aboard the International Space Station, but Scott’s stay was almost twice as long as typical U.S. missions. The continuing investigations are yielding beneficial knowledge on the medical, psychological and biomedical challenges faced by astronauts during long-duration spaceflight.